mouse anti βiii tubulin (Developmental Studies Hybridoma Bank)
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Mouse Anti βiii Tubulin, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 97/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+%CE%B2iii+tubulin/anti-tubulin/pmc13079816-226-79-82
Average 97 stars, based on 29 article reviews
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Binding Assay:Article Title: Stable charged nanobubbles with distinct polarities in culture media differentially affect the viability of human iPSC-derived neurons Article Snippet: Permeabilization was carried out in 0.25% Triton (Nacalai Tesque, Cat# 3550115) in DPBS for 5 min at room temperature, followed by three 5-min times washes in DPBS. .. Non-specific binding of antibodies to the glass surface was blocked in 0.5% fish-skin gelatin in PBS at 37 °C in a humidified 5% CO2 incubator for 1 h. Primary antibodies (PAX6 and SOX2 for NPCs; Tau and βIII-tubulin for neurons) were diluted in the blocking solution at the following concentrations and applied to cells overnight at 4 °C: mouse anti-PAX6 (1:500, Developmental Studies Hybridoma Bank, Cat# PRB-278P), mouse anti-SOX2 (1:200, DSHB, Cat# PCRP-SOX2-1B3), mouse anti-Tau (1:500, DSHB, AB_528487) and Article Title: Stable charged nanobubbles with distinct polarities in culture media differentially affect the viability of human iPSC-derived neurons. Article Snippet: Permeabilization was carried out in 0.25% Triton (Nacalai Tesque, Cat# 3550115) in DPBS for 5 min at room temperature, followed by three 5-min times washes in DPBS. .. Non-specific binding of antibodies to the glass surface was blocked in 0.5% fish-skin gelatin in PBS at 37 °C in a humidified 5% CO2 incubator for 1 h. Primary antibodies (PAX6 and SOX2 for NPCs; Tau and βIII-tubulin for neurons) were diluted in the blocking solution at the following concentrations and applied to cells overnight at 4 °C: mouse anti-PAX6 (1:500, Developmental Studies Hybridoma Bank, Cat# PRB-278P), mouse anti-SOX2 (1:200, DSHB, Cat# PCRP-SOX2-1B3), mouse anti-Tau (1:500, DSHB, AB_528487) and Article Title: Development of charged nanobubble-containing media and their effects on iPSC-derived cell cultures Article Snippet: Permeabilization was carried out in 0.25% Triton (Nacalai Tesque, Cat# 3550115) in DPBS for 5 min at room temperature, followed by three 5-min times washes in DPBS. .. Non-specific binding of antibodies to the glass surface was blocked in 0.5% fish-skin gelatin in PBS at 37 °C in a humidified 5% CO 2 incubator for 1 h. Primary antibodies (PAX6 and SOX2 for NPCs; Tau and βIII-tubulin for neurons) were diluted in the blocking solution at the following concentrations and applied to cells overnight at 4 °C: mouse anti-PAX6 (1:500, Developmental Studies Hybridoma Bank, Cat# PRB-278P), mouse anti-SOX2 (1:200, DSHB, Cat# PCRP-SOX2-1B3), mouse anti-Tau (1:500, DSHB, AB_528487) and Blocking Assay:Article Title: Stable charged nanobubbles with distinct polarities in culture media differentially affect the viability of human iPSC-derived neurons Article Snippet: Permeabilization was carried out in 0.25% Triton (Nacalai Tesque, Cat# 3550115) in DPBS for 5 min at room temperature, followed by three 5-min times washes in DPBS. .. Non-specific binding of antibodies to the glass surface was blocked in 0.5% fish-skin gelatin in PBS at 37 °C in a humidified 5% CO2 incubator for 1 h. Primary antibodies (PAX6 and SOX2 for NPCs; Tau and βIII-tubulin for neurons) were diluted in the blocking solution at the following concentrations and applied to cells overnight at 4 °C: mouse anti-PAX6 (1:500, Developmental Studies Hybridoma Bank, Cat# PRB-278P), mouse anti-SOX2 (1:200, DSHB, Cat# PCRP-SOX2-1B3), mouse anti-Tau (1:500, DSHB, AB_528487) and Article Title: Stable charged nanobubbles with distinct polarities in culture media differentially affect the viability of human iPSC-derived neurons. Article Snippet: Permeabilization was carried out in 0.25% Triton (Nacalai Tesque, Cat# 3550115) in DPBS for 5 min at room temperature, followed by three 5-min times washes in DPBS. .. Non-specific binding of antibodies to the glass surface was blocked in 0.5% fish-skin gelatin in PBS at 37 °C in a humidified 5% CO2 incubator for 1 h. Primary antibodies (PAX6 and SOX2 for NPCs; Tau and βIII-tubulin for neurons) were diluted in the blocking solution at the following concentrations and applied to cells overnight at 4 °C: mouse anti-PAX6 (1:500, Developmental Studies Hybridoma Bank, Cat# PRB-278P), mouse anti-SOX2 (1:200, DSHB, Cat# PCRP-SOX2-1B3), mouse anti-Tau (1:500, DSHB, AB_528487) and Article Title: Development of charged nanobubble-containing media and their effects on iPSC-derived cell cultures Article Snippet: Permeabilization was carried out in 0.25% Triton (Nacalai Tesque, Cat# 3550115) in DPBS for 5 min at room temperature, followed by three 5-min times washes in DPBS. .. Non-specific binding of antibodies to the glass surface was blocked in 0.5% fish-skin gelatin in PBS at 37 °C in a humidified 5% CO 2 incubator for 1 h. Primary antibodies (PAX6 and SOX2 for NPCs; Tau and βIII-tubulin for neurons) were diluted in the blocking solution at the following concentrations and applied to cells overnight at 4 °C: mouse anti-PAX6 (1:500, Developmental Studies Hybridoma Bank, Cat# PRB-278P), mouse anti-SOX2 (1:200, DSHB, Cat# PCRP-SOX2-1B3), mouse anti-Tau (1:500, DSHB, AB_528487) and |
